In the study, twenty-eight peptides were identified in the F < 3 kDa fraction, with five showing antioxidant activity; notably, KPEDWDDR demonstrated the highest affinity for myeloperoxidase and Keap-1.

Oxidative stress arises when prooxidants surpass antioxidants, leading to cellular damage and contributing to health issues such as cancer and cardiovascular diseases.

The polysaccharides improved the lifespan and locomotion abilities of the model organism Caenorhabditis elegans, indicating potential anti-aging effects.

The research employed response surface methodology (RSM) to optimize extraction parameters, revealing significant interactions among solid-to-liquid ratio, extraction temperature, and time.

Additionally, the study optimized the extraction process of crude polysaccharides from the edible mushroom Agrocybe aegerita, evaluating their antioxidant and anti-aging effects.

The research confirms that these hydrolysates do not exhibit cytotoxic effects at the tested concentrations, maintaining 100% cell viability.

Molecular docking was utilized to explore interactions between these identified peptides and target proteins, further elucidating their antioxidant mechanisms.

Antioxidant properties of these extracted polysaccharides were assessed using various scavenging assays, with an IC50 value for superoxide anion scavenging determined at 4.693 mg/mL.

A recent study investigates the cytoprotective effects of hydrolysates derived from the red Californian worm, Eisenia foetida, on differentiated Caco-2 cells, particularly focusing on their ability to mitigate oxidative stress.

The F < 3 kDa fraction exhibited significant cytoprotective activity, enhancing cell viability by 50% against H2O2-induced oxidative stress and restoring redox status.

Optimal extraction conditions were established as a solid-to-liquid ratio yielding a concentration of 0.034 g/mL, an extraction temperature of 92.64 °C, and an extraction time of 2.82 hours, resulting in a yield of 11.325% ± 0.996%.

Findings suggest that low molecular weight peptides from E. foetida could provide therapeutic benefits for conditions related to oxidative stress, highlighting its potential as a source of bioactive peptides.